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The development of new dark field light scattering nanoprobe and dark field imaging application refers to the use of sulfur elements (such as sulfur, selenium, telluride, etc.), etc.), nanomaterials with dark field scattering characteristics, used to enhance the dark field microscope imaging signal, so as to realize the high sensitivity and high resolution of the structure and function of organisms visualization. This nanoprobe has the advantages of low toxicity, high stability, easy synthesis and regulation, and can be used to detect and diagnose various diseases, such as tumor, infection, inflammation, etc. Development of a new dark field light scattering nanoprobe and its case of dark field imaging: Photodynamic therapy and dark field imaging based on non-metrological copper-sulfur nanodrug loading composite: By regulating the ratio of copper and sulfur elements (such as selenium or tellurium), non-metrological copper-sulfur nanomaterials with different optical properties and drug loading capabilities can be prepared for targeted therapy and imaging of tumor cells. Such nanomaterials can produce photothermal effects and reactive oxygen species upon excitation of NIR light, thus enabling photodynamic therapy of tumor cells. Moreover, this nanomaterials can be used as a novel dark-field scattering probe for imaging monitoring of cancer cells during treatment. The results show that the synthetic nano-loaded composite has good prospects for cancer diagnosis and treatment. Bacterium infection detection and dark field imaging based on gold-tellurium nanorod: By modifying tellurium atoms on the surface of gold nanorod, gold-tellurium nanorod with efficient dark field scattering signal and antibacterial activity can be prepared for the detection and imaging of bacterial infections. Such nanorods can specifically bind specifically to proteins on the bacterial surface, enabling a rapid diagnosis of bacterial infection. At the same time, such nanorods can generate a strong scattering signal under dark-field microscopy, thus enabling high-resolution imaging of bacterial infection. Multimodal imaging of tumors based on multifunctional silicon-cadmium sulfide quantum dots: By modifying sulfur-containing polyethylene glycol (PEG) on the surface of silicon quantum dots, multifunctional silicon-cadmium sulfide quantum dots with dual fluorescence emission and dark field scattering characteristics can be prepared for multimodal imaging of tumor cells. Such quantum dots can emit different colored fluorescence signals in UV or near-infrared light, thus enabling fluorescence imaging of tumor cells. At the same time, such quantum dots can produce bright scattering signals under dark field microscopy, thus enabling dark field imaging of tumor cells.