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Storage and transportation
Dry state; -20C protected from light. 6 months. Can be transported at room temperature. Solution: Protect from light at 4°C for 7 days; Protect from light at -20°C for 3 months; Repeated freezing and thawing of the solution will affect the performance of the product, please prepare it for immediate use.
Effective date

See packaging for production date.

Solution preparation
1 Prepare 0.25% (w/v) initiator standard solution
(1) Take 10ml of PBS and add it to a brown bottle filled with initiator LAР {contains 0.025g LAP); (2) Heat and dissolve in a water bath at 40-50°C for 15 minutes, shaking several times during the period.
The LAP standard solution can be stored for 12 months at 4°C and protected from light. 2 Preparation of CMCSMA solution (1) Take the required quality of CMCSMA into the centrifuge tube;
(2 Take the required volume of initiator standard solution and add it to the above centrifuge tube;
(3) Dissolve at 25-50°C for 1-2 hours with constant stirring/shaking during the period; (CMCSMA has high viscosity and longer dissolution time) (4) Sterilize the CMCSMA solution with a 0.22urm sterile syringe filter.
Two-dimensional cell culture recommendations
>Pour the CMCSMA solution into the well plate;
(96-well plate: 50-100uL/well, 46-well plate: 100-300L/well, 24-well plate: 300~500uL/well) Use a 405nm light source and irradiate for 10-30 seconds to gel. It can be lighted Time and intensity control the gel strength; add culture medium to the well to cover the gel, place it in a 37°C incubator for 5 minutes to wash the auspicious product, and aspirate the culture medium;> just add the cell suspension to the well plate. Perform operations such as medium replacement, observation and photography according to the experimental design
(There are no special requirements for operating procedures).

Three-dimensional cell culture recommendations
> Collect cells and resuspend them with CMCSMA solution to prepare cell suspension; add cell suspension to the well plate;
(96-well plate: 50~100L/well, 48-well plate: 100~30QL/well, 24-well plate: 300~500uL/well L) with 405nm light source. Irradiate for 10-30 seconds to gel. The intensity of the gel can be adjusted by the light time and intensity; Tip: Add one hole to solidify one hole to prevent cell precipitation.
>Add culture medium to each well, incubate at 37°C for 5 minutes, wash the sample and remove the culture medium;
Add fresh medium and cultivate for a long time. Perform operations such as culture medium replacement, observation and photographing, immunofluorescence staining, etc. according to the experimental design (no special requirements for operating procedures).
Reminder: Do not look directly at the curing light source.

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